Abstract
In the present investigation, cross-transferability of 125 B. rapa specific and 70 B. nigra specific genomic-SSRs had been evaluated in 16 genotypes of B. carinata (Ethiopian mustard) with an aim to develop genomic resources for Ethiopian mustard. A cross-transferability rate of 82.4% (103 SSRs) and 84.3% (59 SSRs) had been obtained for B. rapa- and B. nigra- derived SSRs, respectively, out of which 66 (64.1%) of B. rapa- and 37 (62.7%) of B. nigra- SSRs resulted into polymorphic amplicons. Comparison of polymorphism parameters including PIC value, gene diversity and heterozygosity values revealed that B. nigra-SSRs were more efficient in terms of transferability rate and polymorphic potential than B. rapa-SSRs. Unweighted pair group method with arithmetic averages-dendrogram divided Ethiopian mustard genotypes into four groups. This newly characterized set of SSR markers will enrich the repertoire of B. carinata SSRs and would provide new paradigms for B. carinata genomics-based improvement.
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The authors wish to thank Indian Council of Agricultural Research (ICAR), New Delhi for funding this work under ICAR-Extra-Mural Research Grant Scheme.
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AKT & KHS formulated the research methodology; DS, NP, LS & JN prepared the draft; DCM performed the data analysis, and AKT & KHS edited and finalized the manuscript.
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Thakur, A.K., Singh, K.H., Sharma, D. et al. Enriching the repertoire of SSR markers of Ethiopian mustard using cross-transferability approach. Plant Physiol. Rep. 27, 65–72 (2022). https://doi.org/10.1007/s40502-021-00639-4
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DOI: https://doi.org/10.1007/s40502-021-00639-4